Real-time polymerase chain reaction (English Wikipedia)

Sambrook, Joseph; Russel, David W. (2001). Molecular Cloning: A Laboratory Manual (3rd ed.). Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press. ISBN 978-0-87969-576-7.

archive.today

Radonic, A; Thulke, S; Mackay, IM; Landt, O; Siegert, W; Nitsche, A (2004). "Guideline for reference gene selection for quantitative real-time PCR". Biochem Biophys Res Commun. 313 (4): 856–862. doi:10.1016/j.bbrc.2003.11.177. PMID 14706621. Archived from the original on 2013-08-02.

cdc.gov

"FDA-cleared RT-PCR Assays and Other Molecular Assays for Influenza Viruses" (PDF). cdc.gov.

doi.org

Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT (2009). "The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments". Clinical Chemistry. 55 (4): 611–622. doi:10.1373/clinchem.2008.112797. PMID 19246619.
Pfaffl, MW; Tichopad, A; Prgomet, C; Neuvians, TP (March 2004). "Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper--Excel-based tool using pair-wise correlations". Biotechnology Letters. 26 (6): 509–15. doi:10.1023/b:bile.0000019559.84305.47. PMID 15127793. S2CID 977404.
Pfaffl, MW; Horgan, GW; Dempfle, L (2002). "Relative Expression Software Tool (REST©) for group wise comparison and statistical analysis of relative expression results in real-time PCR". Nucleic Acids Res. 30 (9): e36. doi:10.1093/nar/30.9.e36. PMC 113859. PMID 11972351.
Vandesompele, J; De Preter, K; Pattyn, F; Poppe, B; Van Roy, N; De Paepe, A; Speleman, F (2002). "Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes". Genome Biology. 3 (7): 1–12. doi:10.1186/gb-2002-3-7-research0034. PMC 126239. PMID 12184808.
Rychlik W, Spencer WJ, Rhoads RE (1990). "Optimization of the annealing temperature for DNA amplification in vitro". Nucleic Acids Res. 18 (21): 6409–6412. doi:10.1093/nar/18.21.6409. PMC 332522. PMID 2243783.
Ponchel F; Toomes C; Bransfield K; Leong F.T; Douglas S.H; Field S.L; Bell S.M; Combaret V; Puisieux A; Mighell A.J (2003). "Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions". BMC Biotechnol. 3: 18. doi:10.1186/1472-6750-3-18. PMC 270040. PMID 14552656.
Ririe K.M; Rasmussen R.P; Wittwer C.T. (1997). "Product Differentiation by Analysis of DNA Melting Curves during the Polymerase Chain Reaction" (PDF). Analytical Biochemistry. 245 (2): 154–160. doi:10.1006/abio.1996.9916. PMID 9056205.
Carr, A. C.; Moore, S. D. (2012). Lucia, Alejandro (ed.). "Robust Quantification of Polymerase Chain Reactions Using Global Fitting". PLOS ONE. 7 (5): e37640. Bibcode:2012PLoSO...737640C. doi:10.1371/journal.pone.0037640. PMC 3365123. PMID 22701526.
Schefe JH, Lehmann KE, Buschmann IR, Unger T, Funke-Kaiser H (2006). "Quantitative real-time RT-PCR data analysis: current concepts and the novel "gene expression's CT difference" formula". J Mol Med. 84 (11): 901–910. doi:10.1007/s00109-006-0097-6. PMID 16972087. S2CID 10427299.
Nailis H, Coenye T, Van Nieuwerburgh F, Deforce D, Nelis HJ (2006). "Development and evaluation of different normalization strategies for gene expression studies in Candida albicans biofilms by real-time PCR". BMC Mol. Biol. 7 (1): 25. doi:10.1186/1471-2199-7-25. PMC 1557526. PMID 16889665.
Nolan T, Hands RE, Bustin SA (2006). "Quantification of mRNA using real-time RT-PCR". Nat. Protoc. 1 (3): 1559–1582. doi:10.1038/nprot.2006.236. PMID 17406449. S2CID 10108148.
Boggy G, Woolf PJ (2010). Ravasi T (ed.). "A Mechanistic Model of PCR for Accurate Quantification of Quantitative PCR Data". PLOS ONE. 5 (8): e12355. Bibcode:2010PLoSO...512355B. doi:10.1371/journal.pone.0012355. PMC 2930010. PMID 20814578.
Ruijter JM, Pfaffl MW, Zhao S, Spiess AN, Boggy G, Blom J, Rutledge RG, Sisti D, Lievens A, De Preter K, Derveaux S, Hellemans J, Vandesompele J (2012). "Evaluation of qPCR curve analysis methods for reliable biomarker discovery: bias, resolution, precision, and implications". Methods. 59 (1): 32–46. doi:10.1016/j.ymeth.2012.08.011. PMID 22975077.
S. Dhanasekaran; T. Mark Doherty; John Kenneth; TB Trials Study Group (March 2010). "Comparison of different standards for real-time PCR-based absolute quantification". Journal of Immunological Methods. 354 (1–2): 34–39. doi:10.1016/j.jim.2010.01.004. PMID 20109462.
Bar, Tzachi; Kubista, Mikael; Tichopad, Ales (2011-10-19). "Validation of kinetics similarity in qPCR". Nucleic Acids Research. 40 (4): 1395–1406. doi:10.1093/nar/gkr778. ISSN 0305-1048. PMC 3287174. PMID 22013160.
Brunner, AM; Yakovlev, IA; Strauss, SH (2004). "Validating internal controls for quantitative plant gene expression studies". BMC Plant Biol. 4: 14. doi:10.1186/1471-2229-4-14. PMC 515301. PMID 15317655.
McGettigan, Paul A (2013). "Transcriptomics in the RNA-seq era". Current Opinion in Chemical Biology. 17 (1): 4–11. doi:10.1016/j.cbpa.2012.12.008. PMID 23290152.
Thellin, O; Zorzi, W; Lakaye, B; De Borman, B; Coumans, B; Henne, G; Grisar, T; Igout, A; Heinen, E (1999). "Housekeeping genes as internal standards: use and limits". J Biotechnol. 75 (2–3): 197–200. doi:10.1016/s0168-1656(99)00163-7. hdl:2268/3661. PMID 10617337.
Radonic, A; Thulke, S; Mackay, IM; Landt, O; Siegert, W; Nitsche, A (2004). "Guideline for reference gene selection for quantitative real-time PCR". Biochem Biophys Res Commun. 313 (4): 856–862. doi:10.1016/j.bbrc.2003.11.177. PMID 14706621. Archived from the original on 2013-08-02.
Dheda, K; Huggett, JF; Bustin, SA; Johnson, MA; Rook, G; Zumla, A (2004). "Validation of housekeeping genes for normalizing RNA expression in real-time PCR". BioTechniques. 37 (1): 112–119. doi:10.2144/04371RR03. PMID 15283208.
Xiu, Leshan; Binder, Raquel A.; Alarja, Natalie A.; Kochek, Kara; Coleman, Kristen K.; Than, Son T.; Bailey, Emily S.; Bui, Vuong N.; Toh, Teck-Hock; Erdman, Dean D.; Gray, Gregory C. (July 2020). "A RT-PCR assay for the detection of coronaviruses from four genera". Journal of Clinical Virology. 128: 104391. doi:10.1016/j.jcv.2020.104391. PMC 7192118. PMID 32403008.
Espy, M.J. (January 2006). "Real-Time PCR in Clinical Microbiology: Applications for Routine Laboratory Testing". Clinical Microbiology Reviews. 19 (3): 165–256. doi:10.1128/CMR.19.1.165-256.2006. PMC 1360278. PMID 16418529.
Dhamad, AE; Abdal Rhida, MA (2020). "COVID-19: molecular and serological detection methods". PeerJ. 8: e10180. doi:10.7717/peerj.10180. PMC 7547594. PMID 33083156.
Bouchez, Blieux, Dequiedt, Domaizon, Dufresne, Ferreira, Godon, Hellal, Joulian, Quaiser, Martin-Laurent, Mauffret, Monier, Peyret, Schmitt-Koplin, Sibourg, D’oiron, Bispo, Deportes, Grand, Cuny, Maron, Ranjard (September 2016). "Molecular Microbiology Methods For Environmental Diagnosis". Environmental Chemistry Letters. 14 (4): 423–441. Bibcode:2016EnvCL..14..423B. doi:10.1007/s10311-016-0581-3. S2CID 88827291. Retrieved 11 May 2020.{{cite journal}}: CS1 maint: multiple names: authors list (link)
Baldwin, B.G. (1992). "Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: An example from the Compositaogy". Molecular Phylogenetics and Evolution. 1 (1): 3–16. Bibcode:1992MolPE...1....3B. doi:10.1016/1055-7903(92)90030-K. PMID 1342921.
Tomlinson, J. A.; Barker, I.; Boonham, N. (2007). "Faster, Simpler, More-Specific Methods for Improved Molecular Detection of Phytophthora ramorum in the Field". Applied and Environmental Microbiology. 73 (12): 4040–4047. Bibcode:2007ApEnM..73.4040T. doi:10.1128/AEM.00161-07. PMC 1932743. PMID 17449689.
Holst-Jensen, Arne; Rønning, Sissel B.; Løvseth, Astrid; Berdal, Knut G. (2003). "PCR technology for screening and quantification of genetically modified organisms (GMOs)". Analytical and Bioanalytical Chemistry. 375 (8): 985–993. doi:10.1007/s00216-003-1767-7. PMID 12733008. S2CID 43681211.
Brodmann P.D; Ilg E.C; Berthoud H; Herrmann A. (2002). "… -Time Quantitative Polymerase Chain Reaction Methods for Four Genetically Modified Maize Varieties …". Journal of AOAC International. 85 (3): 646–653. doi:10.1093/jaoac/85.3.646. PMID 12083257.
Yeh S.H. Tsai C.Y. Kao J.H. Liu C.J. Kuo T.J. Lin M.W. Huang W.L. Lu S.F. Jih J. Chen D.S. Others (2004). "Quantification and genotyping of hepatitis B virus in a single reaction by real-time PCR and melting …". Journal of Hepatology. 41 (4): 659–666. doi:10.1016/j.jhep.2004.06.031. PMID 15464248.
Sawtell N.M. (1998). "The Probability of in Vivo Reactivation of Herpes Simplex Virus Type 1 Increases with the Number of Latently Infected Neurons in the Ganglia". Journal of Virology. 72 (8): 6888–6892. doi:10.1128/JVI.72.8.6888-6892.1998. PMC 109900. PMID 9658140.
Peter M. Rosty C. Couturier J. Radvanyi F. Teshima H. Sastre-garau X. (2006). "MYC activation associated with the integration of HPV DNA at the MYC locus in genital tumours". Oncogene. 25 (44): 5985–5993. doi:10.1038/sj.onc.1209625. PMID 16682952.
Mackay, Ian M.; Arden, Katherine E.; Nitsche, Andreas (2002-03-15). "Real-time PCR in virology". Nucleic Acids Research. 30 (6): 1292–1305. doi:10.1093/nar/30.6.1292. ISSN 0305-1048. PMC 101343. PMID 11884626.

gene-quantification.org

Pfaffl, Michael (2000). "Development and Validation of an Externally Standardized Quantitative Insulin-like Growth Factor-1 RT-PCR Using LightCycler SYBR Green I Technology" (PDF). Biochemica (3) – via gene-quantification.org.

handle.net

hdl.handle.net

Thellin, O; Zorzi, W; Lakaye, B; De Borman, B; Coumans, B; Henne, G; Grisar, T; Igout, A; Heinen, E (1999). "Housekeeping genes as internal standards: use and limits". J Biotechnol. 75 (2–3): 197–200. doi:10.1016/s0168-1656(99)00163-7. hdl:2268/3661. PMID 10617337.

harvard.edu

ui.adsabs.harvard.edu

Carr, A. C.; Moore, S. D. (2012). Lucia, Alejandro (ed.). "Robust Quantification of Polymerase Chain Reactions Using Global Fitting". PLOS ONE. 7 (5): e37640. Bibcode:2012PLoSO...737640C. doi:10.1371/journal.pone.0037640. PMC 3365123. PMID 22701526.
Boggy G, Woolf PJ (2010). Ravasi T (ed.). "A Mechanistic Model of PCR for Accurate Quantification of Quantitative PCR Data". PLOS ONE. 5 (8): e12355. Bibcode:2010PLoSO...512355B. doi:10.1371/journal.pone.0012355. PMC 2930010. PMID 20814578.
Bouchez, Blieux, Dequiedt, Domaizon, Dufresne, Ferreira, Godon, Hellal, Joulian, Quaiser, Martin-Laurent, Mauffret, Monier, Peyret, Schmitt-Koplin, Sibourg, D’oiron, Bispo, Deportes, Grand, Cuny, Maron, Ranjard (September 2016). "Molecular Microbiology Methods For Environmental Diagnosis". Environmental Chemistry Letters. 14 (4): 423–441. Bibcode:2016EnvCL..14..423B. doi:10.1007/s10311-016-0581-3. S2CID 88827291. Retrieved 11 May 2020.{{cite journal}}: CS1 maint: multiple names: authors list (link)
Baldwin, B.G. (1992). "Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: An example from the Compositaogy". Molecular Phylogenetics and Evolution. 1 (1): 3–16. Bibcode:1992MolPE...1....3B. doi:10.1016/1055-7903(92)90030-K. PMID 1342921.
Tomlinson, J. A.; Barker, I.; Boonham, N. (2007). "Faster, Simpler, More-Specific Methods for Improved Molecular Detection of Phytophthora ramorum in the Field". Applied and Environmental Microbiology. 73 (12): 4040–4047. Bibcode:2007ApEnM..73.4040T. doi:10.1128/AEM.00161-07. PMC 1932743. PMID 17449689.

iaea.org

Jawerth, Nicole (27 March 2020). "How is the COVID-19 Virus Detected using real time reverse transcription–polymerase chain reaction?". International Atomic Energy Agency. Retrieved 16 February 2023.

lemuriatechnologies.com

Bruce Gelerter. "PEMF For Treatment Of Corneal Disorders". lemuriatechnologies.com. Archived from the original on 2014-06-09.

lsuhsc.edu

sh.lsuhsc.edu

Ririe K.M; Rasmussen R.P; Wittwer C.T. (1997). "Product Differentiation by Analysis of DNA Melting Curves during the Polymerase Chain Reaction" (PDF). Analytical Biochemistry. 245 (2): 154–160. doi:10.1006/abio.1996.9916. PMID 9056205.

nih.gov

pubmed.ncbi.nlm.nih.gov

Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT (2009). "The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments". Clinical Chemistry. 55 (4): 611–622. doi:10.1373/clinchem.2008.112797. PMID 19246619.
Pfaffl, MW; Tichopad, A; Prgomet, C; Neuvians, TP (March 2004). "Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper--Excel-based tool using pair-wise correlations". Biotechnology Letters. 26 (6): 509–15. doi:10.1023/b:bile.0000019559.84305.47. PMID 15127793. S2CID 977404.
Pfaffl, MW; Horgan, GW; Dempfle, L (2002). "Relative Expression Software Tool (REST©) for group wise comparison and statistical analysis of relative expression results in real-time PCR". Nucleic Acids Res. 30 (9): e36. doi:10.1093/nar/30.9.e36. PMC 113859. PMID 11972351.
Vandesompele, J; De Preter, K; Pattyn, F; Poppe, B; Van Roy, N; De Paepe, A; Speleman, F (2002). "Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes". Genome Biology. 3 (7): 1–12. doi:10.1186/gb-2002-3-7-research0034. PMC 126239. PMID 12184808.
Rychlik W, Spencer WJ, Rhoads RE (1990). "Optimization of the annealing temperature for DNA amplification in vitro". Nucleic Acids Res. 18 (21): 6409–6412. doi:10.1093/nar/18.21.6409. PMC 332522. PMID 2243783.
Ponchel F; Toomes C; Bransfield K; Leong F.T; Douglas S.H; Field S.L; Bell S.M; Combaret V; Puisieux A; Mighell A.J (2003). "Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions". BMC Biotechnol. 3: 18. doi:10.1186/1472-6750-3-18. PMC 270040. PMID 14552656.
Ririe K.M; Rasmussen R.P; Wittwer C.T. (1997). "Product Differentiation by Analysis of DNA Melting Curves during the Polymerase Chain Reaction" (PDF). Analytical Biochemistry. 245 (2): 154–160. doi:10.1006/abio.1996.9916. PMID 9056205.
Carr, A. C.; Moore, S. D. (2012). Lucia, Alejandro (ed.). "Robust Quantification of Polymerase Chain Reactions Using Global Fitting". PLOS ONE. 7 (5): e37640. Bibcode:2012PLoSO...737640C. doi:10.1371/journal.pone.0037640. PMC 3365123. PMID 22701526.
Schefe JH, Lehmann KE, Buschmann IR, Unger T, Funke-Kaiser H (2006). "Quantitative real-time RT-PCR data analysis: current concepts and the novel "gene expression's CT difference" formula". J Mol Med. 84 (11): 901–910. doi:10.1007/s00109-006-0097-6. PMID 16972087. S2CID 10427299.
Nailis H, Coenye T, Van Nieuwerburgh F, Deforce D, Nelis HJ (2006). "Development and evaluation of different normalization strategies for gene expression studies in Candida albicans biofilms by real-time PCR". BMC Mol. Biol. 7 (1): 25. doi:10.1186/1471-2199-7-25. PMC 1557526. PMID 16889665.
Nolan T, Hands RE, Bustin SA (2006). "Quantification of mRNA using real-time RT-PCR". Nat. Protoc. 1 (3): 1559–1582. doi:10.1038/nprot.2006.236. PMID 17406449. S2CID 10108148.
Boggy G, Woolf PJ (2010). Ravasi T (ed.). "A Mechanistic Model of PCR for Accurate Quantification of Quantitative PCR Data". PLOS ONE. 5 (8): e12355. Bibcode:2010PLoSO...512355B. doi:10.1371/journal.pone.0012355. PMC 2930010. PMID 20814578.
Ruijter JM, Pfaffl MW, Zhao S, Spiess AN, Boggy G, Blom J, Rutledge RG, Sisti D, Lievens A, De Preter K, Derveaux S, Hellemans J, Vandesompele J (2012). "Evaluation of qPCR curve analysis methods for reliable biomarker discovery: bias, resolution, precision, and implications". Methods. 59 (1): 32–46. doi:10.1016/j.ymeth.2012.08.011. PMID 22975077.
Overbergh, L.; Giulietti, A.; Valckx, D.; Decallonne, R.; Bouillon, R.; Mathieu, C. (2003). "The use of real-time reverse transcriptase PCR for the quantification of cytokine gene expression". Journal of Biomolecular Techniques. 14 (1): 33–43. PMC 2279895. PMID 12901609.
S. Dhanasekaran; T. Mark Doherty; John Kenneth; TB Trials Study Group (March 2010). "Comparison of different standards for real-time PCR-based absolute quantification". Journal of Immunological Methods. 354 (1–2): 34–39. doi:10.1016/j.jim.2010.01.004. PMID 20109462.
Bar, Tzachi; Kubista, Mikael; Tichopad, Ales (2011-10-19). "Validation of kinetics similarity in qPCR". Nucleic Acids Research. 40 (4): 1395–1406. doi:10.1093/nar/gkr778. ISSN 0305-1048. PMC 3287174. PMID 22013160.
Brunner, AM; Yakovlev, IA; Strauss, SH (2004). "Validating internal controls for quantitative plant gene expression studies". BMC Plant Biol. 4: 14. doi:10.1186/1471-2229-4-14. PMC 515301. PMID 15317655.
McGettigan, Paul A (2013). "Transcriptomics in the RNA-seq era". Current Opinion in Chemical Biology. 17 (1): 4–11. doi:10.1016/j.cbpa.2012.12.008. PMID 23290152.
Thellin, O; Zorzi, W; Lakaye, B; De Borman, B; Coumans, B; Henne, G; Grisar, T; Igout, A; Heinen, E (1999). "Housekeeping genes as internal standards: use and limits". J Biotechnol. 75 (2–3): 197–200. doi:10.1016/s0168-1656(99)00163-7. hdl:2268/3661. PMID 10617337.
Radonic, A; Thulke, S; Mackay, IM; Landt, O; Siegert, W; Nitsche, A (2004). "Guideline for reference gene selection for quantitative real-time PCR". Biochem Biophys Res Commun. 313 (4): 856–862. doi:10.1016/j.bbrc.2003.11.177. PMID 14706621. Archived from the original on 2013-08-02.
Dheda, K; Huggett, JF; Bustin, SA; Johnson, MA; Rook, G; Zumla, A (2004). "Validation of housekeeping genes for normalizing RNA expression in real-time PCR". BioTechniques. 37 (1): 112–119. doi:10.2144/04371RR03. PMID 15283208.
Xiu, Leshan; Binder, Raquel A.; Alarja, Natalie A.; Kochek, Kara; Coleman, Kristen K.; Than, Son T.; Bailey, Emily S.; Bui, Vuong N.; Toh, Teck-Hock; Erdman, Dean D.; Gray, Gregory C. (July 2020). "A RT-PCR assay for the detection of coronaviruses from four genera". Journal of Clinical Virology. 128: 104391. doi:10.1016/j.jcv.2020.104391. PMC 7192118. PMID 32403008.
Espy, M.J. (January 2006). "Real-Time PCR in Clinical Microbiology: Applications for Routine Laboratory Testing". Clinical Microbiology Reviews. 19 (3): 165–256. doi:10.1128/CMR.19.1.165-256.2006. PMC 1360278. PMID 16418529.
Dhamad, AE; Abdal Rhida, MA (2020). "COVID-19: molecular and serological detection methods". PeerJ. 8: e10180. doi:10.7717/peerj.10180. PMC 7547594. PMID 33083156.
Baldwin, B.G. (1992). "Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: An example from the Compositaogy". Molecular Phylogenetics and Evolution. 1 (1): 3–16. Bibcode:1992MolPE...1....3B. doi:10.1016/1055-7903(92)90030-K. PMID 1342921.
Tomlinson, J. A.; Barker, I.; Boonham, N. (2007). "Faster, Simpler, More-Specific Methods for Improved Molecular Detection of Phytophthora ramorum in the Field". Applied and Environmental Microbiology. 73 (12): 4040–4047. Bibcode:2007ApEnM..73.4040T. doi:10.1128/AEM.00161-07. PMC 1932743. PMID 17449689.
Holst-Jensen, Arne; Rønning, Sissel B.; Løvseth, Astrid; Berdal, Knut G. (2003). "PCR technology for screening and quantification of genetically modified organisms (GMOs)". Analytical and Bioanalytical Chemistry. 375 (8): 985–993. doi:10.1007/s00216-003-1767-7. PMID 12733008. S2CID 43681211.
Brodmann P.D; Ilg E.C; Berthoud H; Herrmann A. (2002). "… -Time Quantitative Polymerase Chain Reaction Methods for Four Genetically Modified Maize Varieties …". Journal of AOAC International. 85 (3): 646–653. doi:10.1093/jaoac/85.3.646. PMID 12083257.
Yeh S.H. Tsai C.Y. Kao J.H. Liu C.J. Kuo T.J. Lin M.W. Huang W.L. Lu S.F. Jih J. Chen D.S. Others (2004). "Quantification and genotyping of hepatitis B virus in a single reaction by real-time PCR and melting …". Journal of Hepatology. 41 (4): 659–666. doi:10.1016/j.jhep.2004.06.031. PMID 15464248.
Sawtell N.M. (1998). "The Probability of in Vivo Reactivation of Herpes Simplex Virus Type 1 Increases with the Number of Latently Infected Neurons in the Ganglia". Journal of Virology. 72 (8): 6888–6892. doi:10.1128/JVI.72.8.6888-6892.1998. PMC 109900. PMID 9658140.
Peter M. Rosty C. Couturier J. Radvanyi F. Teshima H. Sastre-garau X. (2006). "MYC activation associated with the integration of HPV DNA at the MYC locus in genital tumours". Oncogene. 25 (44): 5985–5993. doi:10.1038/sj.onc.1209625. PMID 16682952.
Mackay, Ian M.; Arden, Katherine E.; Nitsche, Andreas (2002-03-15). "Real-time PCR in virology". Nucleic Acids Research. 30 (6): 1292–1305. doi:10.1093/nar/30.6.1292. ISSN 0305-1048. PMC 101343. PMID 11884626.

ncbi.nlm.nih.gov

Pfaffl, MW; Horgan, GW; Dempfle, L (2002). "Relative Expression Software Tool (REST©) for group wise comparison and statistical analysis of relative expression results in real-time PCR". Nucleic Acids Res. 30 (9): e36. doi:10.1093/nar/30.9.e36. PMC 113859. PMID 11972351.
Vandesompele, J; De Preter, K; Pattyn, F; Poppe, B; Van Roy, N; De Paepe, A; Speleman, F (2002). "Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes". Genome Biology. 3 (7): 1–12. doi:10.1186/gb-2002-3-7-research0034. PMC 126239. PMID 12184808.
Rychlik W, Spencer WJ, Rhoads RE (1990). "Optimization of the annealing temperature for DNA amplification in vitro". Nucleic Acids Res. 18 (21): 6409–6412. doi:10.1093/nar/18.21.6409. PMC 332522. PMID 2243783.
Ponchel F; Toomes C; Bransfield K; Leong F.T; Douglas S.H; Field S.L; Bell S.M; Combaret V; Puisieux A; Mighell A.J (2003). "Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions". BMC Biotechnol. 3: 18. doi:10.1186/1472-6750-3-18. PMC 270040. PMID 14552656.
Carr, A. C.; Moore, S. D. (2012). Lucia, Alejandro (ed.). "Robust Quantification of Polymerase Chain Reactions Using Global Fitting". PLOS ONE. 7 (5): e37640. Bibcode:2012PLoSO...737640C. doi:10.1371/journal.pone.0037640. PMC 3365123. PMID 22701526.
Nailis H, Coenye T, Van Nieuwerburgh F, Deforce D, Nelis HJ (2006). "Development and evaluation of different normalization strategies for gene expression studies in Candida albicans biofilms by real-time PCR". BMC Mol. Biol. 7 (1): 25. doi:10.1186/1471-2199-7-25. PMC 1557526. PMID 16889665.
Boggy G, Woolf PJ (2010). Ravasi T (ed.). "A Mechanistic Model of PCR for Accurate Quantification of Quantitative PCR Data". PLOS ONE. 5 (8): e12355. Bibcode:2010PLoSO...512355B. doi:10.1371/journal.pone.0012355. PMC 2930010. PMID 20814578.
Overbergh, L.; Giulietti, A.; Valckx, D.; Decallonne, R.; Bouillon, R.; Mathieu, C. (2003). "The use of real-time reverse transcriptase PCR for the quantification of cytokine gene expression". Journal of Biomolecular Techniques. 14 (1): 33–43. PMC 2279895. PMID 12901609.
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